Research Facilities
DEPARTMENT FACILITIES
Fast Protein Liquid Chromatography (FPLC)
Make: M/S GE HEALTHCARE BIOSCIENCES LIMITED. HONGKONG
Model: AKTA Purifier 10
Specification:
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Description | GE Akta Purifier 10 is FPLC system is versatile, modular liquid chromatography systems for fast and reliable separations of proteins, peptides and nucleic acids. |
Flow rate range setting | 0.001 to 10 ml/min |
Pressure range | 0 to 25 MPa |
Conductivity range (RPC – IEX – HIC gradients) |
1 μS/cm to 999.9 mS/cm |
pH range | 2 to 12 |
Temperature range | 4°C to 40°C |
Wavelength range |
190 to 700 nm in steps of 1 nm, 3 wavelengths simultaneously |
Wavelength accuracy | ± 2 nm |
Application | AKTA Purifier 10 is designed for purification of microgram to milligram quantities of proteins. |
Contact Person | Prof. Sharmistha Majumdar |
Freeze Dryer
Make: M/s Labconco Corporation, USA
Model: FreeZone Plus 4.5
Specification:
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Description | The Labconco FreeZone Plus 4.5 Liter Console Freeze Dryer is a compact laboratory lyophilizer with ice holding capacity for light to moderate sample loads. |
Collector Temperature | -84° C, -119° F |
Ice Holding Capacity | 4.5L |
Number of Ports | 12 |
Vacuum Pump | 117 L/ min. Ultimate Vacuum : 2 X 10-3 mBar |
Application | Lyophilization of Proteins, Peptides, Gels etc. |
Contact Person | Prof. Sharad Gupta |
Fluorescence Cell Culture Inverted Microscope
Make: M/s Nikon Corporation, Japan
Model: Eclipse Ts2FL
Specification:
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Description | The ECLIPSE Ts2 FL inverted microscope offers brilliantly clear images, enabling more efficient cell culture observation and documentation. |
Type | Inverted Cell Culture Microscope |
Objectives |
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Eyepiece | TS2-W 10X w/diopter adjustrnent (F.O.V.22mrn) with Diopter Adjuster |
Mechanical Stage with Holders |
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Fluorescence attachments |
Excitation Filter EX 470/40 Dichroic Mirror DM 500 Barrier Filter BA 534/55
Excitation Filter EX 525/50 Dichroic Mirror DM 560 Barrier Filter BA 597/58
Excitation Filter EX 390/38 Dichroic Mirror DM 420 Barrier Filter BA 475/90 |
Camera |
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Application | Routine cell culture studies and imaging. |
Contact Person | Prof. Dhiraj Bhatia |
Gel Documentation System
Make: Bio Rad
Model: Chemidoc MP
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Specification:
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Detector | Cooled CCD, 6 megapixels |
Dynamic range | >4 orders of magnitude |
Illumination source |
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Filter and application |
Standard filter
Chemiluminescence filter
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High Throughput Sequencing setup
Make: M/s Oxford Nanopore Technologie Ltd., UK
Model: Min ION Sequencer (MinION Mk1B)
Specification:
Description | MinION is a powerful, portable sequencing device that delivers cost-effective and real-time access to gigabases of long-read sequencing data. Small enough to fit in a pocket and powerful enough to deliver up to 30 Gb data,the USB-powered MinION allows researchers to rapidly generate actionable biological insights across a wide range of application areas. T |
Size and weight | W 105 mm x H 23 mm x D 33 mm; 100g |
Power | 5 W |
Ports | 1 x USB Type-B |
Environmental conditions | Designed to sequence at 8°C to 25°C* |
Application | The MinION device can be used for DNA and RNA sequencing. |
Contact Person | Prof. Umashankar Singh |
Nanodrop
Make: Thermofisher Scientific
Model: Nanodrop 2000C
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Nanodrop Google form- link
Specification:
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Description | Microvolume Spectrophotometer, with cuvet capability |
Absorbance Accuracy | 3% (at 0.74 Abs at 350 nm) |
Absorbance Range | Pedestal: 0 to 300 A Cuvette: 0 to 1.5 A |
Accuracy | 0.002 |
Detection Limits | Pedestal: 2 ng/μL (dsDNA), 0.10 mg/mL (BSA) Cuvette: 0.4 ng/μL (dsDNA), 0.01 mg/mL (BSA) |
Detection Range | 2 to 15,000 ng/μL (dsDNA), 0.10 to 400 mg/mL (BSA) |
Wavelength Range | 190 to 840 nm |
Volume (Metric) | 0.5 to 2.0 μL |
Measurement Time | >3 sec |
Application | Nucleic Acid Quantification, DNA Quantification, RNA Quantification, Protein Quantification |
Nanoparticle Tracking Analyzer
Make: M/s Particle Metrix GmBH, Germany
Model: PMX 220- 405/488
Specification:
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Description |
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Detection Range | 10 nm – 1000 nm or better |
Concentration range | 106 – 109 particles / mL or better |
Temperature control | Peltier temperature control from ambient – 5oC to 50oC or better. |
Sample Cell | Flow-through chamber with volume 500 uL or less. |
Laser modules/options |
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Application | A broad range of applications from nano-sized metallic particles to polymers and biological nanoparticles such as viruses or extracellular vesicles makes NTA a versatile method for sizing – independent of the refractive index of the particle material. Furthermore, all types of bionanoparticles (BNP) such as protein aggregates, extracellular particles (such as exosomes or EVs), viruses or liposomes can be analyzed and by fluorescence labeling without any additives. |
Contact Person | Prof. Sharad Gupta |
Programmable Research Ultrasound System
Make: M/s Verasonics Inc., USA
Model: VANTAGE 128-Research Ultrasound Platform High Frequency Configuration
Specification:
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Description | Vantage 128 provides researchers and developers a unique, flexible platform for ultrasound innovation across many applications. It is not a clinical ultrasound systemand is used for research purposes only. |
Frequency range |
arbitrary waveform generator) |
Time delay resolution | 4.0 ns |
Programmable pulser voltage | 3.2 to 192 V p-p |
Power limit, single channel | Up to 100 Watts peak, 8 Watts average (into 50 Ohms) |
Transducers |
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Application | Powerful tool for research and development in all applications of ultrasound imaging and non destructive testing. |
Contact Person | Prof. Karla P. Mercado-Shekhar |
Preparative Ultracentrifuge
Make: M/s Beckman Coulter International, USA
Model: Optima XPN 100
Specification:
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Description | The Optima™ XPN Ultracentrifuge from Beckman Coulter Offers a max speed of up to 100,000 rpm. This centrifuge features networking capability and customizable security and tracking to ensure chain of custody and compliance. The Optima XPN offers multi-layered BioSafety features for a safe and productive work environment. |
Maximum speed (rpm) | 1,00,000 |
Maximum rcf (x g) | 8,02,000 |
Speed Control ± rpm of set speed | 1000 |
Set Temperature | 0 to 40°C in 1°C increments |
Accel/Decel Profiles | 10/ 11 |
Available Rotors |
Material :Titanium Max. RPM: 60,000 Max. g-force : 4,85,000, k-factor : 45 6 x 4 ml, Total Volume :24 ml
Material: Titanium Max. rpm: 19000 Max g- force : 53,900 k-factor : 951 6 x 250 ml, Total Volume : 1500 ml
Material: Titanium Max. rpm: 70,000 Max g- force : 504,000 k-factor : 44 8 x 39 ml, Total Volume : 312 ml |
Applications |
Swinging Bucket
Type 19 Rotor
Type 70 Ti
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Contact Person | Prof. Sharad Gupta |
Preparative cum Analytical HPLC system
Make: M/s Shimadzu (Asia Pacific) Pte. Ltd, Singapore
Model: Prominence LC-20 AP
Specification:
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Description | The LC-20AP offers a high level of core performance for a wide range of applications, from analytical to large-scale fractionation. Even though it is especially well-suited to laboratory scale automated continuous fractionation using large-scale preparative columns (20 to 50 mm I.D.), it can also be used with analytical size columns (1 mL/min) for applications ranging from the evaluation of separation conditions and load capacities to validating the purity of fractionated liquids. |
Solvent delivery method | parallel-type double plunger |
Flow rate | 0.01ml/min to 150ml/min without changing pump head |
Flow rate accuracy | ± 1% |
Flow-rate Precision | 0.1% RSD |
Discharge pressure | 6100psi (42 MPa) |
Detectors |
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Application | is used for separating and analyzing organics in a mixture. |
Contact Person | Prof. Sharad Gupta |
Plate Reader
Make: M/s Biotek Instruments Inc., USA
Model: Synergy H1M
Specification:
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Description | Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The Monochromator module (M) supports top and bottom Fluorescence Intensity, UV- Vis absorbance and luminescence detection. |
Absorbance |
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Fluorescence Intensity |
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Time Resolved Fluorescence |
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Luminescence |
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Application | Absorbance, Fluorescence etc. |
Contact Person | Prof. Sharad Gupta |
Real Time PCR
Make: M/s Life Technologies Holding Pte Ltd., Singapore
Model: AB 7500
Specification:
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Description | Applied Biosystems 7500 Real-Time PCR System is a 96-well, five-color platform that uses a fluorescence-based polymerase chain reaction (PCR) reagents to analyze items. Its specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filer sets.. |
Thermal Block Formats | 96 well plate |
Max. Heating Rate (Thermal Block) | 2.5°C/sec. |
Temperature Accuracy (Thermal Block): | 0.25°C (35 to 95°C after 3 min.) |
Detection | 5 emission filters, 5 excitation filters, CCD camera, tungsten-halogen lamp |
Multiplex | Multiplexing |
Sensitivity | 1 Copy of RNase P gene (human genomic DNA) |
Quantitative PCR run time | < 2 hours |
Supported volumes | 20-100 uL |
Application | used for gene expression analysis, pathogen quantitation, SNP genotyping, isothermal, and +/– assays utilizing internal positive controls. |
Contact Person | Prof. Sharad Gupta |
Spectrofluorophotometer
Make: M/s TestRight Nanosystems Pvt. Ltd., New Delhi
Model: TPFA7222022
Specification:
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Description | The instrument is a spectrofluorophotometer with the functionality of both a spectrophotometer and a spectrofluorometer. A fluorometer takes advantage of the fluorescent properties of some compounds to provide information regarding their concentration, properties, and chemical environment in a sample. Whereas a spectrophotometer measures the amount of photons (the intensity of light) absorbed after it passes through the sample solution, giving information about the concentration and absorbance spectra of a sample that can be used for various quantitative analyses. |
Wavelength range | 250-900 nm |
Resolution | 1.8 nm |
Reproducibility | 0.01A |
Lamp | Deuterium Tungsten Lamp |
Detector | UV coated Silicon Linear CCD array |
Fluorescence Upgradable | Yes |
Discrete Excitation module |
Red:600nm Blue:450nm Green:530nm Yellow:575nm |
Sample Support | Liquids, Solids |
Cuvette holder options |
Standard cuvette holder -1cm Shorter Pathlength cuvette -1mm Longer Pathlength cuvette -5cm |
Photometric Accuracy | +0.01A at 0.2A; 10.05A at 1.0A (at 550nm) |
Readout | % T, A, Concentration |
Application | Spectrum, OD, Quantification, Kinetics, Interval |
Contact Person | Prof. Dhiraj Bhatia |
Ultra Microbalance
Make: M/s Radwag, Poland
Model: UYA 2.4Y
Specification:
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Description | 4Y series is a modern weighing device, especially useful when the measurement requires perfect accuracy and high speed. | ||
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2.1 g | ||
Minimum load | 0.01 µg | ||
Readability [d] | 0.1 | ||
Tare range | -2.1 g | ||
Repeatability | 0.3 µg | ||
Linearity | ±1.5 µg | ||
Weighing Pan | 16 mm | ||
Eccentric load deviation | 1.5 µg | ||
Sensitivity stability | 1×10⁻⁶/Year×Rt | ||
Sensitivity offset | 1,5×10⁻⁶×Rt | ||
Sensitivity temperature drift | 1×10⁻⁶/°C×Rt | ||
Minimum weight (USP) | 0.5 mg | ||
Minimum weight (U=1%,k=2) | 0.05 mg | ||
Stabilization time | 10-20 s | ||
Applications | With an impressive readability of 0.0000001g, this microbalance ensures accurate measurements even with minute samples as low as 0.00001g. | ||
Contact Person | Prof. Sharad Gupta |