Research Facilities

DEPARTMENT FACILITIES

Fast Protein Liquid Chromatography (FPLC)

Make: M/S GE HEALTHCARE BIOSCIENCES LIMITED. HONGKONG

Model: AKTA Purifier 10

Specification:

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Description GE Akta Purifier 10 is  FPLC system is versatile, modular liquid chromatography systems for fast and reliable separations of proteins, peptides and nucleic acids. 
Flow rate range setting 0.001 to 10 ml/min
Pressure range 0 to 25 MPa

Conductivity range

(RPC – IEX – HIC gradients)

 1 μS/cm to 999.9 mS/cm
pH range 2 to 12
Temperature range 4°C to 40°C
Wavelength range 

190 to 700 nm in steps of 1 nm,

3 wavelengths simultaneously
Wavelength accuracy  ± 2 nm
Application AKTA Purifier 10 is designed for purification of microgram to milligram quantities of proteins.
Contact Person Prof. Sharmistha Majumdar

Freeze Dryer

Make: M/s Labconco Corporation, USA

Model:  FreeZone Plus 4.5

Specification:

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Description The Labconco FreeZone Plus 4.5 Liter Console Freeze Dryer is a compact laboratory lyophilizer with ice holding capacity for light to moderate sample loads.
Collector Temperature -84° C, -119° F
Ice Holding Capacity 4.5L
Number of Ports 12
Vacuum Pump 117 L/ min. Ultimate Vacuum : 2 X 10-3 mBar
Application Lyophilization of Proteins, Peptides, Gels etc.
Contact Person Prof. Sharad Gupta

Fluorescence Cell Culture Inverted Microscope

Make: M/s Nikon Corporation, Japan

Model:  Eclipse Ts2FL

Specification:

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Description The ECLIPSE Ts2 FL inverted microscope offers brilliantly clear images, enabling more efficient cell culture observation and documentation.
Type Inverted Cell Culture Microscope
Objectives
  • CFI Achromat 4X A, N.A. 0. 10,                       W.D. 30.00 mm
  • CFI Achromat ADL 10X B N.A. 0.25.              W.D. 6.2mm. PH- 1
  • CFI Achromat LWD ADL 20XF N.A. 0.40, W.D. 3.1 mm, PH- 1
  • CFI Achromat LWD ADL,40XF N.A. 0.55, W.D, 2.1 mm, PH -1
Eyepiece TS2-W 10X w/diopter adjustrnent (F.O.V.22mrn) with Diopter Adjuster
Mechanical Stage with Holders
  • TS2-S-SM Mechanical Stage (with handle) Cross travel : 126 (X) x 78 (Y) mm
  •  C-S-HT Terasaki holder
  •  C-S-HS Slide Glass Flolder
  •  C-S-HP35 Petridish Holder 35mm
Fluorescence attachments
  • C-LED470 Epi- FL Filter Cube consisting of

Excitation Filter EX 470/40

Dichroic Mirror DM 500

Barrier Filter BA 534/55

 

  • C-LED525 Epi- FL Filter Cube consisting of

Excitation Filter EX 525/50

Dichroic Mirror DM 560

Barrier Filter BA 597/58

 

  • C-LED385 Epi-FL Filter Cube consisting of

Excitation Filter EX 390/38

Dichroic Mirror DM 420

Barrier Filter BA 475/90
Camera
  • 1/1.8 inch Color CMOS image sensor        Size: 6.91 x 4.92 mm
Application Routine cell culture studies and imaging.
Contact Person Prof. Dhiraj Bhatia

Gel Documentation System

Make: Bio Rad
Model: Chemidoc MP

Gel Documentation system booking calendar-  link

Gel Documentation system Google form- link

Specification:

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Detector Cooled CCD, 6 megapixels
Dynamic range >4 orders of magnitude
Illumination source
  • Trans-UV, 302 nm excitation
  • Epi-white
  • Trans-white (requires White Sample Tray)
  • Trans-blue, 450−490 nm excitation (requires Blue SampleTray)
  • Epi-blue, 460−490 nm excitation
  • Epi-green, 520−545 nm excitation
  • Epi-red, 625−650 nm excitation
  • Epi-far red, 650−675 nm excitation
  • Epi-near IR, 755−777 nm excitation
Filter and application

Standard filter

  • 590/110 nm standard filter to perform protein and DNA gel and blot imaging

Chemiluminescence filter

  • 518–546 nm filter for blue-excitable fluorophores and stains
  • 577–613 nm filter for green-excitable fluorophores and stains
  • 675–725 nm for red-excitable fluorophores and stains
  • 700–730 nm filter for far red-excitable fluorophores and stains
  • 813–860 nm for near IR-excitable fluorophores and stains

High Throughput Sequencing setup

Make: M/s Oxford Nanopore Technologie Ltd., UK

Model: Min ION Sequencer (MinION Mk1B)

Specification:

Description MinION is a powerful, portable sequencing device that delivers cost-effective and real-time access to gigabases of long-read sequencing data. Small enough to fit in a pocket and powerful enough to deliver up to 30 Gb data,the USB-powered MinION allows researchers to rapidly generate actionable biological insights across a wide range of application areas. T
Size and weight W 105 mm x H 23 mm x D 33 mm; 100g
Power 5 W
Ports 1 x USB Type-B
Environmental conditions  Designed to sequence at 8°C to 25°C*
Application The MinION device can be used for DNA and RNA sequencing.
Contact Person Prof. Umashankar Singh

Nanodrop

Make: Thermofisher Scientific
Model: Nanodrop 2000C

Nanodrop booking calendar- link

Nanodrop Google form- link

Specification:

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Description Microvolume Spectrophotometer, with cuvet capability
Absorbance Accuracy 3% (at 0.74 Abs at 350 nm)
Absorbance Range Pedestal: 0 to 300 A Cuvette: 0 to 1.5 A
Accuracy 0.002
Detection Limits Pedestal: 2 ng/μL (dsDNA), 0.10 mg/mL (BSA)
Cuvette: 0.4 ng/μL (dsDNA), 0.01 mg/mL (BSA)
Detection Range 2 to 15,000 ng/μL (dsDNA), 0.10 to 400 mg/mL (BSA)
Wavelength Range 190 to 840 nm
Volume (Metric) 0.5 to 2.0 μL
Measurement Time >3 sec
Application Nucleic Acid Quantification, DNA Quantification, RNA Quantification, Protein Quantification

 

Nanoparticle Tracking Analyzer

Make: M/s Particle Metrix GmBH, Germany

Model: PMX 220- 405/488

Specification:

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Description
  • Laser scattering based detection of nanoparticles in solution integrated with microscopic particle visualization.
  • Direct observation of individual particles and measurement of diffusion events to yield high-resolution nanoparticle size distribution and concentration.
Detection Range 10 nm – 1000 nm or better
Concentration range 106 – 109 particles / mL or better
Temperature control Peltier temperature control from ambient – 5oC to 50oC or better.
Sample Cell Flow-through chamber with volume 500 uL or less.
Laser modules/options
  • Two lasers: 405 nm and 488 nm along with Appropriate fluorescence filters to cover the range of excitation wavelengths.
Application A broad range of applications from nano-sized metallic particles to polymers and biological nanoparticles such as viruses or extracellular vesicles makes NTA a versatile method for sizing – independent of the refractive index of the particle material. Furthermore, all types of bionanoparticles (BNP) such as protein aggregates, extracellular particles (such as exosomes or EVs), viruses or liposomes can be analyzed and by fluorescence labeling without any additives.
Contact Person Prof. Sharad Gupta

Programmable Research Ultrasound System

Make: M/s Verasonics Inc., USA

Model: VANTAGE 128-Research Ultrasound Platform High Frequency Configuration

Specification:

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Description Vantage 128  provides researchers and developers a unique, flexible platform for ultrasound innovation across many applications. It is not a clinical ultrasound systemand is used for research purposes only.
Frequency range
  • 1 to 50 MHz with High Frequency configuration*(Also includes a programmable high pass filter and

arbitrary waveform generator)
Time delay resolution 4.0 ns
Programmable pulser voltage 3.2 to 192 V p-p
Power limit, single channel Up to 100 Watts peak, 8 Watts average (into 50 Ohms)
Transducers
  • Verasonics L11-5v linear array transducer
  • Verasonics L35-16vX high frequency linear array transducer
Application Powerful tool for research and development in all applications of ultrasound imaging and non destructive testing.
Contact Person Prof. Karla P. Mercado-Shekhar

Preparative Ultracentrifuge

Make: M/s Beckman Coulter International, USA

Model: Optima XPN 100

Specification:

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Description The Optima™ XPN Ultracentrifuge from Beckman Coulter Offers a max speed of up to 100,000 rpm. This centrifuge features networking capability and customizable security and tracking to ensure chain of custody and compliance. The Optima XPN offers multi-layered BioSafety features for a safe and productive work environment.
Maximum speed (rpm) 1,00,000
Maximum rcf (x g) 8,02,000
Speed Control  ± rpm of set speed 1000
Set Temperature 0 to 40°C in 1°C increments
Accel/Decel Profiles  10/ 11
Available Rotors
  • SW 60 Ti Rotor Package Swing out

Material :Titanium 

Max. RPM: 60,000 

Max. g-force : 4,85,000, 

k-factor : 45

6 x 4 ml, Total Volume :24 ml

 

  • Type 19 Rotor assembly Fixed angle

Material: Titanium

Max. rpm: 19000

Max g- force : 53,900

k-factor : 951

6 x 250 ml, Total Volume : 1500 ml

 

  • Type 70 Ti  Rotor assembly Fixed-Angle

Material: Titanium

Max. rpm: 70,000

Max g- force : 504,000

k-factor : 44

8 x 39 ml, Total Volume : 312 ml
Applications

Swinging Bucket

  • Rate-zonal separation of subcellular organelles, pelleting and isopycnic separations of RNA.

Type 19 Rotor

  • Differential centrifugation of large volumes of viruses and large subcellular particles.

Type 70 Ti

  • Differential centrifugation of subcellular fractions.
Contact Person Prof. Sharad Gupta

Preparative cum Analytical HPLC system

Make: M/s Shimadzu (Asia Pacific) Pte. Ltd, Singapore

Model: Prominence LC-20 AP

Specification:

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Description The LC-20AP offers a high level of core performance for a wide range of applications, from analytical to large-scale fractionation. Even though it is especially well-suited to laboratory scale automated continuous fractionation using large-scale preparative columns (20 to 50 mm I.D.), it can also be used with analytical size columns (1 mL/min) for applications ranging from the evaluation of separation conditions and load capacities to validating the purity of fractionated liquids.
Solvent delivery method parallel-type double plunger
Flow rate 0.01ml/min to 150ml/min without changing pump head
Flow rate accuracy ± 1%
Flow-rate Precision 0.1% RSD
Discharge pressure 6100psi (42 MPa)
Detectors
  1. Photo Diode Array (PDA) Detector
  • Light Source: Deuterium (D2) lamp & Tungsten Lamp
  • Wavelength range: 190 to 800 nm
  • Bandwidth: Selectable (High Resolution- 1,.2nm/High Sensitivity-8nm)
  • Wavelength accuracy: ± 1 nm
  • Number of diode elements: 512
  • Noise Level: 0.6×10-s AU 
  • Drift: 5x1O-4 AU/h

 

  1. Refractive Index Detector 
  • Refractive index range: 1to 1.75 RIU
  • Noise : 2.5×10-9 RIU
  • Drift: 1×10-7 RIU/hour
  • Temperature control to the cell unit through oven 30 to 60oC
  • Flow Cell Volume: 9ul
Application is used for separating and analyzing organics in a mixture.
Contact Person Prof. Sharad Gupta

Plate Reader

Make: M/s Biotek Instruments Inc., USA
Model: Synergy H1M

Specification:

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Description Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The Monochromator module (M) supports top and bottom Fluorescence Intensity, UV- Vis absorbance and luminescence detection.
Absorbance
  • Light Source : Xenon Flash Lamp
  • Wavelength Selection: Monochromator
  • Wavelength range: 230 -999 nm in 1 nm increment
  • Bandpass : 4nm (230- 285 nm) , 8 nm (> 285 nm)
  • Dynamic range : 0 to 4.0 OD
  • Resolution : 0.0001 OD
Fluorescence Intensity
  • Light Source : Xenon Flash Lamp
  • Wavelength Selection: Quadruple Grating Monochromator for both top and bottom reading
  • Wavelength range: 250 – 700 nm 
  • Sensitivity with Monochromator: Top- Fluorescein 2.5 pM typical (0.25 fmol/384 well plate)
  • Bottom Fluorescein  4.0 pM typical (0.4 fmol/384 well plate)
  • Detection system – PMT 
Time Resolved Fluorescence
  • Light Source : Xenon Flash Lamp
  • Wavelength range: 250 – 700 nm
  • Sensitivity : Monos Europium 120 amol/well, 384 well plate
Luminescence
  • Wavelength range: 300 – 700 nm 
  • Sensitivity : 20 amol ATP typical(flash)
Application Absorbance, Fluorescence  etc.
Contact Person Prof. Sharad Gupta

Real Time PCR

Make:  M/s Life Technologies Holding Pte Ltd., Singapore

Model: AB 7500

Specification:

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Description Applied Biosystems 7500 Real-Time PCR System is a 96-well, five-color platform that uses a fluorescence-based polymerase chain reaction (PCR) reagents to analyze items. Its specialized optical system enables easy and accurate calibration to new dyes without requiring the addition of new filer sets..
Thermal Block Formats 96 well plate
Max. Heating Rate (Thermal Block) 2.5°C/sec.
Temperature Accuracy (Thermal Block):  0.25°C (35 to 95°C after 3 min.)
Detection 5 emission filters, 5 excitation filters, CCD camera, tungsten-halogen lamp
Multiplex Multiplexing
Sensitivity 1 Copy of RNase P gene (human genomic DNA)
Quantitative PCR run time < 2 hours
Supported volumes 20-100 uL
Application used for gene expression analysis, pathogen quantitation, SNP genotyping, isothermal, and +/– assays utilizing internal positive controls.
Contact Person Prof. Sharad Gupta

Spectrofluorophotometer

Make: M/s TestRight Nanosystems Pvt. Ltd., New Delhi

Model: TPFA7222022

Specification:

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Description The instrument is a spectrofluorophotometer with the functionality of both a spectrophotometer and a spectrofluorometer. A fluorometer takes advantage of the fluorescent properties of some compounds to provide information regarding their concentration, properties, and chemical environment in a sample. Whereas a spectrophotometer measures the amount of photons (the intensity of light) absorbed after it passes through the sample solution, giving information about the concentration and absorbance spectra of a sample that can be used for various quantitative analyses.
Wavelength range 250-900 nm 
Resolution  1.8 nm
Reproducibility  0.01A 
Lamp  Deuterium Tungsten Lamp
Detector UV coated Silicon Linear CCD array 
Fluorescence Upgradable Yes
Discrete Excitation module 

Red:600nm 

Blue:450nm 

Green:530nm 

Yellow:575nm 
Sample Support  Liquids, Solids 
Cuvette holder options 

Standard cuvette holder -1cm 

Shorter Pathlength cuvette -1mm 

Longer Pathlength cuvette -5cm
Photometric Accuracy  +0.01A at 0.2A; 10.05A at 1.0A (at 550nm)
Readout  % T, A, Concentration 
Application Spectrum, OD, Quantification, Kinetics, Interval 
Contact Person Prof. Dhiraj Bhatia

Ultra Microbalance

Make: M/s Radwag, Poland

Model: UYA 2.4Y

Specification:

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Description 4Y series is a modern weighing device, especially useful when the measurement requires perfect accuracy and high speed.

 

Max capacity (max)
2.1 g
Minimum load 0.01 µg
Readability [d] 0.1 
Tare range -2.1 g
Repeatability 0.3 µg
Linearity ±1.5 µg
Weighing Pan 16 mm
Eccentric load deviation 1.5 µg
Sensitivity stability 1×10⁻⁶/Year×Rt 
Sensitivity offset 1,5×10⁻⁶×Rt
Sensitivity temperature drift 1×10⁻⁶/°C×Rt
Minimum weight (USP) 0.5 mg
Minimum weight (U=1%,k=2) 0.05 mg
Stabilization time 10-20 s
Applications With an impressive readability of 0.0000001g, this microbalance ensures accurate measurements even with minute samples as low as 0.00001g.
Contact Person Prof. Sharad Gupta

Central Instrumentation Facility

SAFETY

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